Physicochemical analysis of rotavirus segment 11 supports a 'modified panhandle' structure and not the predicted alternative tRNA-like structure (TRLS)

.Rotaviruses are a major cause of acute gastroenteritis, which is often fatal in infants. The viral genome consists of 11 double-stranded RNA segments, but little is known about their cis-acting sequences and structural elements. Covariation studies and phylogenetic analysis exploring the potential structure of RNA11 of rotaviruses suggested that, besides the previously predicted "modified panhandle" structure, the 5' and 3' termini of one of the isoforms of the bovine rotavirus UKtc strain may interact to form a tRNA-like structure (TRLS). Such TRLSs have been identified in RNAs of plant viruses, where they are important for enhancing replication and packaging. However, using tRNA mimicry assays (in vitro aminoacylation and 3'- adenylation), we found no biochemical evidence for tRNA-like functions of RNA11. Capping, synthetic 3' adenylation and manipulation of divalent cation concentrations did not change this finding. NMR studies on a 5'- and 3'-deletion construct of RNA11 containing the putative intra-strand complementary sequences supported a predominant panhandle structure and did not conform to a cloverleaf fold despite the strong evidence for a predicted structure in this conserved region of the viral RNA. Additional viral or cellular factors may be needed to stabilise it into a form with tRNA-like properties

Genomic analysis of codon, sequence and structural conservation with selective biochemical-structure mapping reveals highly conserved and dynamic structures in rotavirus RNAs with potential cis-acting functions

Rotaviruses are a major cause of acute, often fatal, gastroenteritis in infants and young children world-wide. Virions contain an 11 segment double-stranded RNA genome. Little is known about the cis-acting sequences and structural elements of the viral RNAs. Using a database of 1621 full-length sequences of mammalian group A rotavirus RNA segments, we evaluated the codon, sequence and RNA structural conservation of the complete genome. Codon conservation regions were found in eight ORFs, suggesting the presence of functional RNA elements. Using ConStruct and RNAz programmes, we identified conserved secondary structures in the positive-sense RNAs including long-range interactions (LRIs) at the 5′ and 3′ terminal regions of all segments. In RNA9, two mutually exclusive structures were observed suggesting a switch mechanism between a conserved terminal LRI and an independent 3′ stem–loop structure. In RNA6, a conserved stem–loop was found in a region previously reported to have translation enhancement activity. Biochemical structural analysis of RNA11 confirmed the presence of terminal LRIs and two internal helices with high codon and sequence conservation. These extensive in silico and in vitro analyses provide evidence of the conservation, complexity, multi-functionality and dynamics of rotavirus RNA structures which likely influence RNA replication, translation and genome packaging

Gender, Power and Sexual Abuse in the Pacific: Rev Simpson’s Improper Liberties

In 1843 on the island of Tahiti the evangelical missionary Rev. Alexander Simpson was accused of sexually assaulting three of the female students under his care, and of taking 'improper liberties' with at least three more. The events did not come out in public for at least a decade, while Simpson's power in the local community only grew and rumblings relating to his wrong-doings were ruthlessly 'crushed'. By exploring the case of Rev. Simpson, Emily Manktelow gives us key insights into the gender, power and racial dynamics of a particular case of sexual abuse on the frontiers of European colonialism. She explores the social and sexual context of clerical abuse, considers the hierarchies of gender and power that determined how the case was handled, investigates the nature of colonialism, gender and abuse in the 19th century. The uncomfortably timely content of Gender, Power and Sexual Abuse in the Pacific is uncomfortably timely allows us to interrogate the way we deal with and represent issues of abuse, authority and childhood in the modern world. It aims to give voice to those whom the archive has silenced, and to listen to what they have to tell us about gender, sexuality and abuse in the modern world

Subverting Empire: Deviance and Disorder in the British Colonial World

The British Empire was never as orderly as its architects would have us believe. Across the British imperial world, rules were broken, norms and social conventions were ignored and boundaries were transgressed. This is the first historical study to probe the colonial history of deviance, bringing to light stories of subversive behaviour that were deliberately covered up. With case studies ranging from Britain to New Zealand, India to East and Southern Africa, the book reveals what deviance in a colonial context actually entailed, as well as the ways in which deviants themselves were categorised, controlled and concealed. Ranging from murder to madness, forgery to fornication, Subverting Empire shows up the diverse ways in which governments attempted to enforce social order – and the ingeniousness of those who undermined it

Reverend Simpson’s “Improper Liberties”: Moral Scrutiny and Missionary Children in the South Seas Mission

This article explores a case of alleged sexual abuse, by a missionary, of a number of missionary daughters in the South Seas mission. The rich material connected with this case allows for a powerful insight into the fabric of life of missionaries and their children, and illuminates various levels of moral scrutiny and social supervision that were embedded in the mission context. Parental moral scrutiny was the most effective mechanism to neutralise the internal threat posed by the potential deviance of missionary children. The levels of moral scrutiny in the mission were unevenly implemented, however, and the asymmetry of scrutiny that occurred (whereby missionary children were subjected to intense social and moral supervision, while missionaries themselves were permitted a certain level of deviation from spiritual and cultural norms) reflected the hierarchies of power and social dynamics of the mission context. This case led to the systemisation and legitimisation of both these scrutinies and these asymmetries, while further implementing new levels of social control, directed both at the behaviour of missionary children and the efficiency of parental moral scrutiny enacted by their parents

Thinking with gossip: deviance, rumour and reputation in the South Seas Mission of the London Missionary Society

Recalling his arrival at the island of Tahiti, site of the London Missionary Society’s South Seas Mission (SSM) in 1842, Reverend John Jesson could not help noting his dismay at the prevalence of gossip among his missionary brethren